Lab Technician I - Laboratory
New Jersey, United States of America
Full Time
1 day ago
About this role
Laboratory Technician
Job Summary: Medical Diagnostic Laboratories(MDL), a member of Genesis Biotechnology Group, is a CLIA certified clinical laboratory with multiple state licensing, specializing in state of the art, automated DNA based molecular analysis of a variety of chronic and infectious illnesses.
MDL specializes and performs Polymerase Chain Reaction (PCR) with a larger menu of testing available in the field of infectious disease. Our main theme of research is in the field of Gynecology, Infectious Diseases, Infectious Arthritis, Tick-borne Diseases, Mycology, and Chronic Fatigue Syndrome (CFS).
MDL is looking for a Laboratory Technician. The Laboratory Technicians responsible for printing labels and attaching requisitions to the corresponding specimens.
Essential Functions:
Accessioning:
▪ Print labels and attach to requisitions and the corresponding specimens
▪ Properly accession multiple specimen types
▪ Take photographs of all specimens received
▪ Properly identify any discrepant information
▪ File all paperwork in order and distribute to the appropriate QC bins
▪ Properly scan accessioned requisitions
▪ Scan 2-D barcode elution tubes used for extraction
▪ Properly locate and record temperatures for all necessary stations
Manual Extraction:
▪ Perform Phenol/Chloroform manual extractions on biopsies and ticks
▪ Properly separate samples into their organic and inorganic layers via phenol/chloroform
▪ Properly perform quantitative analysis and hydrate all ticks and biopsies
Robotic Extraction:
▪ Properly extract all samples types using a provided XG sheet as a template
▪ Properly set up liquid handling robotics for each extracted XG sheet with the correct volumes of all necessary reagents
▪ Properly dispose of all waste material when required; both hazardous and non-hazardous
▪ Attend to robotics to address any user interactions and/or robotic malfunctions
▪ Manually extract all necessary samples via commercial kits
RNA Extraction:
▪ Perform manual RNA extractions via Qiagen kit for all necessary samples
▪ Perform robotic RNA extractions via VX Reagents kit for all necessary samples
PCR:
▪ Perform manual and robotic Conventional method PCR amplification
▪ Properly load and start thermocycler machines
▪ Perform manual and robotic Real-time method PCR amplification
▪ Properly load and start Rotor-gene thermocycler
▪ Perform initial analysis on completed Real-time PCR
▪ Perform Acoustic Real-Time PCR method for amplification with the aid of the ATS-100 Acoustic Dispensor
▪ Properly load and start Bio-Rad CFX thermocycler
▪ Perform initial analysis on completed Acoustic Real-Time PCR
Gel Electrophoresis:
▪ Properly identify which completed PCRs from the thermocycler require gel electrophoresis
▪ Properly identify when to make and/or use 2% versus 2.5% gels
▪ Complete blank gel template sheets to combine short PCR sheets
▪ Use dye to stain all necessary samples with the appropriate volume on a test by test basis
▪ Properly load correct agarose gel with the appropriate volume of ladder and sample; in addition properly identify the appropriate settings on which to run each gel (i.e. voltage & time)
▪ Properly capture a visual image of the gel with the use of a SYNGENE UV gel reader
▪ Be able to troubleshoot any gel box or power source errors, as well as any gel reader errors
Pyrosequencing
▪ Properly perform nested PCR prior to sequencing
▪ Properly load and run Sequencing Analysis reactions (SQA)
▪ Properly load and run Single Nucleotide Polymorphism reactions (SNP)
▪ Properly analyze all run data
Micro-Biology/Cell Culture
▪ Inoculate appropriate plate with swab media; for Candida species, Urinary Pathogens, GBS, Enterococcus species, and the Wound Panel; Streak for single colonies
▪ Properly determine specific species present, and inoculate appropriate plate with said species on a quad plate preloaded with antibiotics, or an agar plate stamped with antibiotic discs
▪ Properly analyze growth plate to determine antibiotic susceptibility or resistance
▪ For GBS Antibiotic Resistance testing, properly assemble rack and operate Anaerobic Chamber System
▪ Identify and pick colonies for PCR
▪ Perform CAMP test and analyze for zones of hemolysis
Next-Generation Sequencing
▪ Properly perform a series of nested PCRs for BRCA, HPV, and Cystic Fibrosis
▪ Properly attach samples to appropriate bead mixtures
▪ Properly perform beads cleaning to remove unused dNTP’s and primers within the amplified PCR product
▪ Properly perform gel purification with the use of the Pippin Prep machine
▪ Properly load and operate multiple Life Technologies Equipment
▪ Properly analyze all run data
Additional Responsibilities:
▪ Performs other similar or related duties as required or assigned.
▪ Assures activities are conducted in a safe manner and materials are handled according to safety guidelines.
▪ Assures customer privacy is maintained at all times.
▪ Participates as a team member and is accountable for own work responsibilities.
▪ Assures information is delivered in a manner that is supportive, timely and understandable.
▪ Initiative is demonstrated to proactively diagnose and resolve problems.
▪ Productive work habits are consistently displayed.
▪ Accuracy and quality are maintained for all activities.
Job Qualifications: General Knowledge, Skills, and Abilities (KSA’s) required
▪ 9-month Clinical Laboratory Technician training
Physical Demands: Physical, Mental and Workplace Environment Conditions
▪ Ability to sit, stand, walk and perform repetitive motion (manual pipette)
▪ Proficient visual and audio skills
Workplace Conditions: Workplace Environment Conditions
▪ Laboratory setting
▪ Use of personal protective equipment may be necessary
▪ May be exposed to chemicals, blood/body fluids
Education and Certifications:
• Bachelor Degree in Biology, Chemistry, or related science field
We Offer:
• Competitive Salary
• Medical, Dental & Vision Insurance
• Short and Long Term Disability
• Life Insurance
• Dependent Care Flex Spending Account
• Voluntary Policies (Accident, Hospital Indemnity, Critical Illness & Supplemental STD)
• Identity Theft Protection
• 401(k) Plan
• Paid Vacation & Holiday
• Business Casual Environment
Visit us at https://www.genesisglobalgrp.com/
Genesis Biotechnology Group is a member of Genesis Global Group, an equal opportunity employer.
Job Summary: Medical Diagnostic Laboratories(MDL), a member of Genesis Biotechnology Group, is a CLIA certified clinical laboratory with multiple state licensing, specializing in state of the art, automated DNA based molecular analysis of a variety of chronic and infectious illnesses.
MDL specializes and performs Polymerase Chain Reaction (PCR) with a larger menu of testing available in the field of infectious disease. Our main theme of research is in the field of Gynecology, Infectious Diseases, Infectious Arthritis, Tick-borne Diseases, Mycology, and Chronic Fatigue Syndrome (CFS).
MDL is looking for a Laboratory Technician. The Laboratory Technicians responsible for printing labels and attaching requisitions to the corresponding specimens.
Essential Functions:
Accessioning:
▪ Print labels and attach to requisitions and the corresponding specimens
▪ Properly accession multiple specimen types
▪ Take photographs of all specimens received
▪ Properly identify any discrepant information
▪ File all paperwork in order and distribute to the appropriate QC bins
▪ Properly scan accessioned requisitions
▪ Scan 2-D barcode elution tubes used for extraction
▪ Properly locate and record temperatures for all necessary stations
Manual Extraction:
▪ Perform Phenol/Chloroform manual extractions on biopsies and ticks
▪ Properly separate samples into their organic and inorganic layers via phenol/chloroform
▪ Properly perform quantitative analysis and hydrate all ticks and biopsies
Robotic Extraction:
▪ Properly extract all samples types using a provided XG sheet as a template
▪ Properly set up liquid handling robotics for each extracted XG sheet with the correct volumes of all necessary reagents
▪ Properly dispose of all waste material when required; both hazardous and non-hazardous
▪ Attend to robotics to address any user interactions and/or robotic malfunctions
▪ Manually extract all necessary samples via commercial kits
RNA Extraction:
▪ Perform manual RNA extractions via Qiagen kit for all necessary samples
▪ Perform robotic RNA extractions via VX Reagents kit for all necessary samples
PCR:
▪ Perform manual and robotic Conventional method PCR amplification
▪ Properly load and start thermocycler machines
▪ Perform manual and robotic Real-time method PCR amplification
▪ Properly load and start Rotor-gene thermocycler
▪ Perform initial analysis on completed Real-time PCR
▪ Perform Acoustic Real-Time PCR method for amplification with the aid of the ATS-100 Acoustic Dispensor
▪ Properly load and start Bio-Rad CFX thermocycler
▪ Perform initial analysis on completed Acoustic Real-Time PCR
Gel Electrophoresis:
▪ Properly identify which completed PCRs from the thermocycler require gel electrophoresis
▪ Properly identify when to make and/or use 2% versus 2.5% gels
▪ Complete blank gel template sheets to combine short PCR sheets
▪ Use dye to stain all necessary samples with the appropriate volume on a test by test basis
▪ Properly load correct agarose gel with the appropriate volume of ladder and sample; in addition properly identify the appropriate settings on which to run each gel (i.e. voltage & time)
▪ Properly capture a visual image of the gel with the use of a SYNGENE UV gel reader
▪ Be able to troubleshoot any gel box or power source errors, as well as any gel reader errors
Pyrosequencing
▪ Properly perform nested PCR prior to sequencing
▪ Properly load and run Sequencing Analysis reactions (SQA)
▪ Properly load and run Single Nucleotide Polymorphism reactions (SNP)
▪ Properly analyze all run data
Micro-Biology/Cell Culture
▪ Inoculate appropriate plate with swab media; for Candida species, Urinary Pathogens, GBS, Enterococcus species, and the Wound Panel; Streak for single colonies
▪ Properly determine specific species present, and inoculate appropriate plate with said species on a quad plate preloaded with antibiotics, or an agar plate stamped with antibiotic discs
▪ Properly analyze growth plate to determine antibiotic susceptibility or resistance
▪ For GBS Antibiotic Resistance testing, properly assemble rack and operate Anaerobic Chamber System
▪ Identify and pick colonies for PCR
▪ Perform CAMP test and analyze for zones of hemolysis
Next-Generation Sequencing
▪ Properly perform a series of nested PCRs for BRCA, HPV, and Cystic Fibrosis
▪ Properly attach samples to appropriate bead mixtures
▪ Properly perform beads cleaning to remove unused dNTP’s and primers within the amplified PCR product
▪ Properly perform gel purification with the use of the Pippin Prep machine
▪ Properly load and operate multiple Life Technologies Equipment
▪ Properly analyze all run data
Additional Responsibilities:
▪ Performs other similar or related duties as required or assigned.
▪ Assures activities are conducted in a safe manner and materials are handled according to safety guidelines.
▪ Assures customer privacy is maintained at all times.
▪ Participates as a team member and is accountable for own work responsibilities.
▪ Assures information is delivered in a manner that is supportive, timely and understandable.
▪ Initiative is demonstrated to proactively diagnose and resolve problems.
▪ Productive work habits are consistently displayed.
▪ Accuracy and quality are maintained for all activities.
Job Qualifications: General Knowledge, Skills, and Abilities (KSA’s) required
▪ 9-month Clinical Laboratory Technician training
Physical Demands: Physical, Mental and Workplace Environment Conditions
▪ Ability to sit, stand, walk and perform repetitive motion (manual pipette)
▪ Proficient visual and audio skills
Workplace Conditions: Workplace Environment Conditions
▪ Laboratory setting
▪ Use of personal protective equipment may be necessary
▪ May be exposed to chemicals, blood/body fluids
Education and Certifications:
• Bachelor Degree in Biology, Chemistry, or related science field
We Offer:
• Competitive Salary
• Medical, Dental & Vision Insurance
• Short and Long Term Disability
• Life Insurance
• Dependent Care Flex Spending Account
• Voluntary Policies (Accident, Hospital Indemnity, Critical Illness & Supplemental STD)
• Identity Theft Protection
• 401(k) Plan
• Paid Vacation & Holiday
• Business Casual Environment
Visit us at https://www.genesisglobalgrp.com/
Genesis Biotechnology Group is a member of Genesis Global Group, an equal opportunity employer.